Recombinant Anti-Flag Tag antibody (HRP Conjugated)-标签抗体,直标抗体-科普欣生物 Cookbio 抗体|仪器|耗材|试剂-武汉科普欣生物科技有限公司

Recombinant Anti-Flag Tag antibody (HRP Conjugated)

咨询

￥980

加入对比

货号： KZ547817-HRP-100

别名： DDDDK tag, DYKDDDDK, ddk, DDDDK epitope tag, ECS epitope tag, Enterokinase Cleavage Site tag, FLAG tag, FLAG

应用 | 种属

WB Species independent

规格：

100 μL

产品描述
应用
背景
储存条件
图像

产品信息

蛋白质全称	Flag标签
别名	DDDDK tag, DYKDDDDK, ddk, DDDDK epitope tag, ECS epitope tag, Enterokinase Cleavage Site tag, FLAG tag, FLAG
免疫原	KLH偶联的DYKDDDDK合成肽
纯化方式	亲和纯化

应用

WB 免疫印迹

物种:Species independent

稀释比:1: 2000-1: 5000

阳性样品:

背景

The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.产品颜色为浅黄色.

图像