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Recombinant Anti-His Tag antibody (兔单克隆抗体)
¥980
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货号: K5453765-100
别名: 6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His probe, His Tag, His-probe, HISTIDINE TAGGED
应用 | 种属
WB Species independent
ICC/IF Species independent
规格:
产品信息
蛋白质全称 His标签
别名 6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His probe, His Tag, His-probe, HISTIDINE TAGGED
免疫原 KLH偶联的HHHHHH (6* His)合成肽
抗体亚型 IgG, κ
克隆号 SB81
纯化方式 亲和纯化
应用
WB 免疫印迹
物种:Species independent
稀释比:1: 2000-1: 5000
阳性样品:
ICC/IF 免疫细胞化学/免疫荧光
物种:Species independent
稀释比:1: 500-1: 1000
阳性样品:
背景
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His-tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His-tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.
储存条件
储存条件:储存条件在:-20℃下储存一年, 避免反复冻融.
储存缓冲液:含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.
图像
His Tag_d2000_WB

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