Anti-His Tag 兔多克隆抗体-标签抗体-科普欣生物 Cookbio 抗体|仪器|耗材-武汉科普欣生物科技有限公司

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Anti-His Tag 兔多克隆抗体

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￥980

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货号： K1333753-100

别名： 6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His probe, His Tag, His-probe, HISTIDINE TAGGED

应用 | 种属

WB Species independent

规格：

100 μL

产品描述
应用
背景
储存条件
图像

产品信息

蛋白质全称	His标签
别名	6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His probe, His Tag, His-probe, HISTIDINE TAGGED
Uniprot ID	None
免疫原	KLH偶联的HHHHHH (6* His)合成肽
抗体亚型	IgG
纯化方式	亲和纯化

应用

WB 免疫印迹

物种:Species independent

稀释比:1: 500-1: 1000

阳性样品:

背景

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His-tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His-tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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