Anti-Leptin 兔多克隆抗体-脂代谢,激素及受体-科普欣生物 Cookbio 抗体|仪器|耗材|试剂-武汉科普欣生物科技有限公司

Anti-Leptin 兔多克隆抗体

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￥1900

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货号： K1338772-100

预测分子量： 19 kDa

观测分子量： 19 kDa

别名： LEPD,leptin,LEP,leptin,OB,Obese protein,Obesity factor,OBS

应用 | 种属

WB H,M,R

规格：

100 μL 50 μL

产品描述
应用
背景
储存条件
图像

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产品信息

蛋白质全称	瘦素
别名	LEPD,leptin,LEP,leptin,OB,Obese protein,Obesity factor,OBS
Uniprot ID	P41159
免疫原	人Leptin重组蛋白
抗体亚型	IgG
纯化方式	亲和纯化
预测分子量./观测分子量.	19 kDa / 19 kDa

应用

WB 免疫印迹

物种:人,小鼠,大鼠

稀释比:1:500-1:1000

阳性样品:睾丸, 结肠

背景

The protein is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism.

储存条件

储存条件：储存条件在：-20℃下储存一年, 避免反复冻融.

储存缓冲液：含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

图像

WB检测Leptin蛋白(货号 K1338772).
样品: 经RIPA裂解液(货号KSG2002)处理的蛋白质.
封闭: 3%脱脂牛奶(货号KSGC310001)溶于TBST溶液, 室温孵育1小时.
—抗: 1: 1000稀释, 4℃ 孵育过夜.
二抗: HRP标记山羊抗兔IgG (H+L) (货号KB69909), 1: 5000稀释, 室温孵育1小时.